Reactive species
Human;Mouse
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Histone H2B type F-S
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5. AA range:1-50
Specificity
Acetyl-Histone H2B (K5) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K5.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s;H2BK5AC
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element.
Function
DNA packaging, chromatin organization, chromatin assembly or disassembly, nucleosome assembly, defense response, response to bacterium, chromatin assembly, cellular macromolecular complex subunit organization, cellular macromolecular complex assembly, nucleosome organization, defense response to bacterium, macromolecular complex subunit organization, chromosome organization, macromolecular complex assembly, protein-DNA complex assembly,
