Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Protein name
Fatty acid-binding protein, intestinal
Immunogen
Purified recombinant fragment of human I-FABP expressed in E. Coli.
Specificity
I-FABP Monoclonal Antibody detects endogenous levels of I-FABP protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
FABP2; FABPI; Fatty acid-binding protein; intestinal; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP
Background
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008],
Function
domain:Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.,function:FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.,induction:By EGF.,similarity:Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.,tissue specificity:Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.,
