Reactive species
Human;Mouse;Monkey
Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Protein name
ATP-binding cassette sub-family G member 2
Immunogen
Purified recombinant fragment of human ABCG2 expressed in E. Coli.
Specificity
ABCG2 Monoclonal Antibody detects endogenous levels of ABCG2 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
ABCG2; ABCP; BCRP; BCRP1; MXR; ATP-binding cassette sub-family G member 2; Breast cancer resistance protein; CDw338; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter; CD antigen CD338
Background
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene.
Function
function:Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.,induction:Up-regulated in brain tumors.,PTM:Glycosylation-deficient ABCG2 is normally expressed and functional.,similarity:Belongs to the ABC transporter family. ABCG (White) subfamily.,similarity:Contains 1 ABC transmembrane type-2 domain.,similarity:Contains 1 ABC transporter domain.,subunit:Monomer or homodimer; disulfide-linked.,tissue specificity:Highly expressed in placenta. Low expression in small i
