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Apaf-1 Polyclonal Antibody

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Apaf-1 Polyclonal Antibody

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Product code: YP-Ab-00576

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse;Rat

Applications

WB;IHC;IF;ELISA

Antibody type

Polyclonal Antibody

Gene Name

APAF1

Protein name

Apoptotic protease-activating factor 1

Dalton（DA）

135kD

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550

Specificity

Apaf-1 Polyclonal Antibody detects endogenous levels of Apaf-1 protein.

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Polyclonal, Rabbit,IgG

Dilution rate

WB: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000.. IF 1:50-200

Purification process

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Stockpile

-20°C/1 year

Other name

APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Function

domain:The CARD domain mediates interaction with APIP.,function:Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.,induction:By E2F and p53 in apoptotic neurons.,similarity:Contains 1 CARD domain.,similarity:Contains 1 NB-ARC domain.,similarity:Contains 13 WD repeats.,subunit:Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP.,tissue specificity:Ubiquitous. Highest levels of expression in adult spleen and per

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

WB

蛋白免疫印迹（WB) 实验步骤

Apaf-1 Polyclonal Antibody

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Apaf-1 Polyclonal Antibody

Product introduction

Product Details

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Citation

Background information

FAQ

Experimental scheme

IP

WB

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