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Rad17 Polyclonal Antibody

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Rad17 Polyclonal Antibody

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Product code: YP-Ab-00504

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse

Applications

WB;IHC;IF;ELISA

Antibody type

Polyclonal Antibody

Gene Name

RAD17

Protein name

Cell cycle checkpoint protein RAD17

Dalton（DA）

86kD

Immunogen

The antiserum was produced against synthesized peptide derived from human RAD17. AA range:621-670

Specificity

Rad17 Polyclonal Antibody detects endogenous levels of Rad17 protein.

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Polyclonal, Rabbit,IgG

Dilution rate

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification process

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Stockpile

-20°C/1 year

Other name

RAD17; R24L; Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, h

Function

function:Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.,induction:By X-ray irradiation (isoform 1, isoform 3 and isoform 4).,PTM:Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or

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IP

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WB

蛋白免疫印迹（WB) 实验步骤

Rad17 Polyclonal Antibody

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Rad17 Polyclonal Antibody

Product introduction

Product Details

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Citation

Background information

FAQ

Experimental scheme

IP

WB

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