Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Cytochrome b5
Immunogen
The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110
Specificity
Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5
Background
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],
Function
disease:Defects in CYB5A are the cause of type IV hereditary methemoglobinemia [MIM:250790].,function:Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.,similarity:Belongs to the cytochrome b5 family.,similarity:Contains 1 cytochrome b5 heme-binding domain.,
