Reactive species
Human;Mouse;Rat
Applications
WB;IHC;IF;ELISA
Antibody type
Polyclonal Antibody
Protein name
Apoptosis-associated speck-like protein containing a CARD
Immunogen
The antiserum was produced against synthesized peptide derived from human ASC. AA range:10-59
Specificity
ASC Polyclonal Antibody detects endogenous levels of ASC protein.
Constitute
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Polyclonal, Rabbit,IgG
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Other name
PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
Background
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
domain:Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.,function:Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase 9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.,miscellaneous:In breast tumorigenesis, methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death.,PTM:Phosphorylated.,similarity:Contains 1 CARD domain.,similarity:Contains 1 DAPIN domain.,subcellular location:Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.,subunit:Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PY
