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CAD (phospho-Ser1859) rabbit pAb

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CAD (phospho-Ser1859) rabbit pAb

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Product code: YP-Ab-00266

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse;Rat

Applications

Antibody type

Polyclonal Antibody

Gene Name

CAD

Protein name

CAD (Ser1859)

Dalton（DA）

250kD

Immunogen

Synthesized phosho peptide around human CAD (Ser1859)

Specificity

This antibody detects endogenous levels of Human Mouse Rat CAD (phospho-Ser1859)

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Polyclonal, Rabbit,IgG

Dilution rate

WB 1:1000-2000

Purification process

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Stockpile

-20°C/1 year

Other name

CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase (EC 6.3.5.5); Aspartate carbamoyltransferase (EC 2.1.3.2); Dihydroorotase (EC 3.5.2.3)]

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],

Function

catalytic activity:(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.,catalytic activity:2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.,catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) .,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction.,function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).,online information:Aspartate carbamoyltransferase entry,pathway:Pyrimi

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

WB

蛋白免疫印迹（WB) 实验步骤

CAD (phospho-Ser1859) rabbit pAb

Product navigation

CAD (phospho-Ser1859) rabbit pAb

Product introduction

Product Details

Customer data and reviews (0)

Citation

Background information

FAQ

Experimental scheme

IP

WB

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