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Caspase-6 (phospho Ser257) Polyclonal Antibody

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Caspase-6 (phospho Ser257) Polyclonal Antibody

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Product code: YP-Ab-00150

Promotional price: $ 255

100UL 53UL 10UL

Product introduction

Reactive species

Human;Mouse;Rat

Applications

WB;IHC;IF;ELISA

Antibody type

Polyclonal Antibody

Gene Name

CASP6

Protein name

Caspase6

Dalton（DA）

15,30kD

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 6 around the phosphorylation site of Ser257. AA range:223-272

Specificity

Phospho-Caspase-6 (S257) Polyclonal Antibody detects endogenous levels of Caspase-6 protein only when phosphorylated at S257.

Constitute

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Source

Polyclonal, Rabbit,IgG

Dilution rate

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification process

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Stockpile

-20°C/1 year

Other name

CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015],

Function

catalytic activity:Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.,enzyme regulation:Activation is suppressed by phosphorylation at Ser-257.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.,PTM:Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.,similarity:Belongs to the peptidase C14A family.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit.,

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IP

常规Immunoprecipitation（免疫沉淀 IP）实验操作步骤

WB

蛋白免疫印迹（WB) 实验步骤

Caspase-6 (phospho Ser257) Polyclonal Antibody

Product navigation

Caspase-6 (phospho Ser257) Polyclonal Antibody

Product introduction

Product Details

Customer data and reviews (0)

Citation

Background information

FAQ

Experimental scheme

IP

WB

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