Reactive species
Human;Rat
Applications
WB;IHC;IF;FCM;ELISA
Antibody type
Monoclonal antibodies
Protein name
RING finger and CHY zinc finger domain-containing protein 1
Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Specificity
Pirh2 Monoclonal Antibody detects endogenous levels of Pirh2 protein.
Constitute
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/10000. Not yet tested in other applications.
Purification process
Affinity purification
Other name
RCHY1; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; RING finger and CHY zinc finger domain-containing protein 1; Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger
Background
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013],
Function
similarity:Contains 1 CHY-type zinc finger.,similarity:Contains 1 CTCHY-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with AR, PLAG1 and SCYL1BP1/NTKLBP1.,
