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Rat Nuclear Factor κB Subunit p65 (NFKB-p65) Detection Kit

Product code: SYP-R0188
Promotional price: $ 458

Product introduction

Reactive species
Rat
Examination area
156.25-5000pg/mL
Sensitivity
Physical properties
Each liquid component should be clear and transparent, without precipitation or floccules. The aluminum foil bag of the microplate should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of rat nuclear factor κB subunit p65 (NFKB-p65) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-rat nuclear factor κB subunit p65 (NFKB-p65) antibody (solid phase antibody), add rat nuclear factor κB subunit p65 (NFKB-p65) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of rat nuclear factor κB subunit p65 (NFKB-p65) in the sample to be tested. By fitting the calibration curve, the concentration of rat nuclear factor κB subunit p65 (NFKB-p65) in the sample can be calculated.
Specificity
This kit recognizes native rat nuclear factor κB subunit p65 (NFKB-p65) and has no cross-reactivity with structural analogs.

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