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Human α-smooth muscle actin (α-SMA) detection kit

Product code: SYP-H4195
Promotional price: $ 458

Product introduction

Reactive species
people
Examination area
400pg/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of human α-smooth muscle actin (α-SMA) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-human α-smooth muscle actin (α-SMA) antibody (solid phase antibody), human α-smooth muscle actin (α-SMA) calibrator and the sample to be tested are added, and then biotin-labeled antibody is added. After incubation and sufficient washing, HRP-coupled avidin is added. After incubation and sufficient washing, unbound components are removed, and a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme is formed on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA reader. The absorbance (OD value) is positively correlated with the concentration of human α-smooth muscle actin (α-SMA) in the sample to be tested. The concentration of human α-smooth muscle actin (α-SMA) in the sample can be calculated by fitting the calibrator curve.
Specificity
This kit recognizes native human α-smooth muscle actin (α-SMA) with no cross-reactivity with structural analogs.

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