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Human sequence similar family 19 member A5 (FAM19A5) detection kit

Product code: SYP-H3782
Promotional price: $ 458

Product introduction

Reactive species
people
Examination area
78-5,000pg/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of human sequence similar family 19 member A5 (FAM19A5) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-human sequence similar family 19 member A5 (FAM19A5) antibody (solid phase antibody), add human sequence similar family 19 member A5 (FAM19A5) calibrator and the sample to be tested, then add biotin-labeled antibody, after incubation and sufficient washing, add HRP-coupled avidin, after incubation and sufficient washing, remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of human sequence similar family 19 member A5 (FAM19A5) in the sample to be tested. By fitting the calibration curve, the concentration of human sequence similar family 19 member A5 (FAM19A5) in the sample can be calculated.
Specificity
This kit recognizes the natural human sequence similarity family 19 member A5 (FAM19A5) and has no crossover with structural analogs.

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