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Human B-cell lymphoma factor 9-like protein (Bcl9L) detection kit

Product code: SYP-H3435
Promotional price: $ 458

Product introduction

Reactive species
people
Examination area
0.312-20ng/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of human B-cell lymphoma factor 9-like protein (Bcl9L) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In the microwell ELISA plate pre-coated with anti-human B-cell lymphoma factor 9-like protein (Bcl9L) antibody (solid phase antibody), add human B-cell lymphoma factor 9-like protein (Bcl9L) calibrator and the sample to be tested, then add biotin-labeled antibody, after incubation and sufficient washing, add HRP-coupled avidin, after incubation and sufficient washing, remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of human B-cell lymphoma factor 9-like protein (Bcl9L) in the sample to be tested. The concentration of human B-cell lymphoma factor 9-like protein (Bcl9L) in the sample can be calculated by fitting the calibration curve.
Specificity
This kit recognizes native human B-cell lymphoma factor 9-like protein (Bcl9L) and has no cross-reactivity with structural analogs.

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