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Human Ubiquitin-Conjugating Enzyme E2C (UBE2C) Detection Kit

Product code: SYP-H1565
Promotional price: $ 458

Product introduction

Reactive species
people
Examination area
0.156-10 ng/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of human ubiquitin-conjugating enzyme E2C (UBE2C) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-human ubiquitin conjugating enzyme E2C (UBE2C) antibody (solid phase antibody), add human ubiquitin conjugating enzyme E2C (UBE2C) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of human ubiquitin conjugating enzyme E2C (UBE2C) in the sample to be tested. Fitting the calibrator curve can calculate the concentration of human ubiquitin conjugating enzyme E2C (UBE2C) in the sample.
Specificity
This kit recognizes native human ubiquitin-conjugating enzyme E2C (UBE2C) with no crosstalk with structural analogs.

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