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Human osteoprotegerin (OPG) detection kit

Product code: SYP-H0310
Promotional price: $ 458

Product introduction

Reactive species
people
Examination area
0.3125-10 ng/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of human osteoprotegerin (OPG) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Human osteoprotegerin (OPG) calibrator and the sample to be tested are added to the microwell ELISA plate pre-coated with anti-human osteoprotegerin (OPG) antibody (solid phase antibody), and then the biotin-labeled antibody is added. After incubation and sufficient washing, HRP-coupled avidin is added. After incubation and sufficient washing, the unbound components are removed, and a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme is formed on the solid surface of the microwell plate. TMB colorimetric solution is added to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the microplate reader. The absorbance (OD value) is positively correlated with the concentration of human osteoprotegerin (OPG) in the sample to be tested. The concentration of human osteoprotegerin (OPG) in the sample can be calculated by fitting the calibrator curve.
Specificity
This kit recognizes natural human osteoprotegerin (OPG) and has no cross-reactivity with structural analogs.

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