Home / Products / Human ELISA kit /

Human Carbohydrate Antigen 19-9 (CA19-9) Detection Kit

Product code: SYP-H0188
Promotional price: $ 458

Product introduction

Reactive species
people
Examination area
12.5-400 IU/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of human carbohydrate antigen 19-9 (CA19-9) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-human carbohydrate antigen 19-9 (CA19-9) antibody (solid phase antibody), human carbohydrate antigen 19-9 (CA19-9) calibrator and the sample to be tested are added, and then biotin-labeled antibody is added. After incubation and sufficient washing, HRP-coupled avidin is added. After incubation and sufficient washing, unbound components are removed, and a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme is formed on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of human carbohydrate antigen 19-9 (CA19-9) in the sample to be tested. The concentration of human carbohydrate antigen 19-9 (CA19-9) in the sample can be calculated by fitting the calibrator curve.
Specificity
This kit recognizes the natural human carbohydrate antigen 19-9 (CA19-9) and has no cross-reactivity with structural analogs.

Open

Product Details

Customer data and reviews (0)

Fold content

Citation

Fold content

Experimental scheme

Fold content
>