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Chicken cellar protein (CAV1) detection kit

Product code: SYP-C0110
Promotional price: $ 458

Product introduction

Reactive species
chicken
Examination area
0.156-10 ng/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of chicken caveolin (CAV1) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-chicken caveolin (CAV1) antibody (solid phase antibody), chicken caveolin (CAV1) calibrator and the sample to be tested are added, and then biotin-labeled antibody is added. After incubation and sufficient washing, HRP-coupled avidin is added. After incubation and sufficient washing, unbound components are removed, and a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme is formed on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of chicken caveolin (CAV1) in the sample to be tested. Fitting the calibrator curve can calculate the concentration of chicken caveolin (CAV1) in the sample.
Specificity
This kit recognizes native chicken caveolin (CAV1) and has no cross-talk with structural analogs.

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