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Bovine Apolipoprotein B100 (APOB100) Detection Kit

Product code: SYP-B0091
Promotional price: $ 458

Product introduction

Reactive species
ox
Examination area
18.75-1200ng/mL
Sensitivity
Physical properties
Each liquid component is clear and transparent, without precipitation or floccules. Microplate aluminum foil bagIt should be vacuum packed without damage or leakage.
Use
Used to detect the concentration of bovine apolipoprotein B100 (APOB100) in samples such as serum, plasma, and cell culture supernatant.
Experimental principle
This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-bovine apolipoprotein B100 (APOB100) antibody (solid phase antibody), add bovine apolipoprotein B100 (APOB100) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of bovine apolipoprotein B100 (APOB100) in the sample to be tested. Fitting the calibrator curve can calculate the concentration of bovine apolipoprotein B100 (APOB100) in the sample.
Specificity
This kit recognizes native bovine apolipoprotein B100 (APOB100) and has no cross-reactivity with structural analogs.

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